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Structure paper

TitleA threonine stabilizes the NiC and NiR catalytic intermediates of [NiFe]-hydrogenase.
Journal, issue, pagesJ. Biol. Chem., Vol. 290, Page 8550-8558, Year 2015
Publish dateDec 4, 2014 (structure data deposition date)
AuthorsAbou-Hamdan, A. / Ceccaldi, P. / Lebrette, H. / Gutierrez-Sanz, O. / Richaud, P. / Cournac, L. / Guigliarelli, B. / De Lacey, A.L. / Leger, C. / Volbeda, A. ...Abou-Hamdan, A. / Ceccaldi, P. / Lebrette, H. / Gutierrez-Sanz, O. / Richaud, P. / Cournac, L. / Guigliarelli, B. / De Lacey, A.L. / Leger, C. / Volbeda, A. / Burlat, B. / Dementin, S.
External linksJ. Biol. Chem. / PubMed:25666617
MethodsX-ray diffraction
Resolution1.95 - 2.6 Å
Structure data

PDB-4ucq:
Structure of the T18D small subunit mutant of D. fructosovorans NiFe- hydrogenase
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-4ucw:
Structure of the T18V small subunit mutant of D. fructosovorans NiFe- hydrogenase
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-4ucx:
Structure of the T18G small subunit mutant of D. fructosovorans NiFe- hydrogenase
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-F3S:
FE3-S4 CLUSTER

ChemComp-GOL:
GLYCEROL

ChemComp-FCO:
CARBONMONOXIDE-(DICYANO) IRON

ChemComp-NI:
NICKEL (II) ION

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

Source
  • desulfovibrio fructosivorans (bacteria)
KeywordsOXIDOREDUCTASE / NIFE-HYDROGENASE MUTANT / UNREADY STATE / NIFE-HYDROGENASE / T18G MUTANT

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