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Structure paper

TitleRationally engineered flavin-dependent oxidase reveals steric control of dioxygen reduction.
Journal, issue, pagesFebs J., Vol. 282, Page 3060-3074, Year 2015
Publish dateMar 17, 2014 (structure data deposition date)
AuthorsZafred, D. / Steiner, B. / Teufelberger, A.R. / Hromic, A. / Karplus, P.A. / Schofield, C.J. / Wallner, S. / Macheroux, P.
External linksFebs J. / PubMed:25619330
MethodsX-ray diffraction
Resolution1.3 - 2.3 Å
Structure data

PDB-4pve:
Wild-type Phl p 4.0202, a glucose dehydrogenase
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-4pvh:
Phl p 4 N158H variant, a glucose dehydrogenase
Method: X-RAY DIFFRACTION / Resolution: 1.4 Å

PDB-4pvj:
Phl p 4 I153V variant, a glucose oxidase
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-4pvk:
Phl p 4 I153V N158H variant, a glucose oxidase
Method: X-RAY DIFFRACTION / Resolution: 1.3 Å

PDB-4pwb:
Phl p 4 I153V variant, a glucose oxidase, pressurized with Xenon
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-4pwc:
Phl p 4 I153V N158H variant, a glucose oxidase, 3.5 M NaBr soak
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-4pzf:
Berberine bridge enzyme G164A variant, a reticuline dehydrogenase
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-NA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-MLI:
MALONATE ION

ChemComp-HOH:
WATER

ChemComp-XE:
XENON

ChemComp-BR:
BROMIDE ION

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-SO4:
SULFATE ION

ChemComp-12P:
DODECAETHYLENE GLYCOL / precipitant*YM

Source
  • phleum pratense (timothy grass)
  • eschscholzia californica (California poppy)
KeywordsOXIDOREDUCTASE / FLAVOPROTEIN / BI-COVALENT FLAVINYLATION / ALLERGEN / GLUCOSE DEHYDROGENASE / LOW OXYGEN REACTIVITY / GRASS POLLEN / GLUCOSE OXIDASE / HIGH OXYGEN REACTIVITY / Reticuline oxidase / Berberine bridge-forming enzyme / Tetrahydroprotoberberine synthase

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