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Structure paper

TitleStructural Characterization of O- and C-Glycosylating Variants of the Landomycin Glycosyltransferase LanGT2.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 54, Page 2811-2815, Year 2015
Publish dateOct 6, 2014 (structure data deposition date)
AuthorsTam, H.K. / Harle, J. / Gerhardt, S. / Rohr, J. / Wang, G. / Thorson, J.S. / Bigot, A. / Lutterbeck, M. / Seiche, W. / Breit, B. ...Tam, H.K. / Harle, J. / Gerhardt, S. / Rohr, J. / Wang, G. / Thorson, J.S. / Bigot, A. / Lutterbeck, M. / Seiche, W. / Breit, B. / Bechthold, A. / Einsle, O.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:25581707
MethodsX-ray diffraction
Resolution1.85 - 2.22 Å
Structure data

PDB-4rie:
Landomycin Glycosyltransferase LanGT2
Method: X-RAY DIFFRACTION / Resolution: 2.162 Å

PDB-4rif:
Landomycin Glycosyltransferase LanGT2, carbasugar substrate complex
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-4rig:
Chimeric Glycosyltransferase LanGT2S8Ac
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-4rih:
Chimeric Glycosyltransferase LanGT2S8Ac, carbasugar substrate complex
Method: X-RAY DIFFRACTION / Resolution: 2.22 Å

PDB-4rii:
Chimeric Glycosyltransferase LanGT2S8Ac, TDP complex
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-3R2:
2'-deoxy-5'-O-[(R)-{[(R)-{[(1S,3R,4R,5S)-3,4-dihydroxy-5-methylcyclohexyl]oxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]-3,4-dihydrothymidine

ChemComp-MG:
Unknown entry

ChemComp-TYD:
THYMIDINE-5'-DIPHOSPHATE

Source
  • streptomyces cyanogenus (bacteria)
  • streptomyces fradiae (bacteria)
KeywordsTRANSFERASE / GT fold / glycosyltransferase / C-glycosylating glycosyltransferase

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