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Structure paper

TitleMolecular insights into RNA and DNA helicase evolution from the determinants of specificity for a DEAD-box RNA helicase.
Journal, issue, pagesElife, Vol. 3, Page e04630-e04630, Year 2014
Publish dateJul 8, 2014 (structure data deposition date)
AuthorsMallam, A.L. / Sidote, D.J. / Lambowitz, A.M.
External linksElife / PubMed:25497230
MethodsX-ray diffraction
Resolution2.197 - 3.209 Å
Structure data

PDB-4tyn:
DEAD-box helicase Mss116 bound to ssDNA and ADP-BeF
Method: X-RAY DIFFRACTION / Resolution: 2.959 Å

PDB-4tyw:
DEAD-box helicase Mss116 bound to ssRNA and ADP-BeF
Method: X-RAY DIFFRACTION / Resolution: 2.197 Å

PDB-4tyy:
DEAD-box helicase Mss116 bound to ssRNA and CDP-BeF
Method: X-RAY DIFFRACTION / Resolution: 2.74 Å

PDB-4tz0:
DEAD-box helicase Mss116 bound to ssRNA and GDP-BeF
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

PDB-4tz6:
DEAD-box helicase Mss116 bound to ssRNA and UDP-BeF
Method: X-RAY DIFFRACTION / Resolution: 3.209 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-BEF:
BERYLLIUM TRIFLUORIDE ION

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-HOH:
WATER

ChemComp-CDP:
CYTIDINE-5'-DIPHOSPHATE

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-UDP:
URIDINE-5'-DIPHOSPHATE / UDP*YM

Source
  • saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
KeywordsHYDROLASE/DNA / DEAD-box / RNA helicase / hydrolase / RNA BINDING PROTEIN-DNA complex / HYDROLASE-DNA complex / RNA BINDING PROTEIN/RNA / RNA BINDING PROTEIN-RNA complex

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