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Structure paper

TitleStructural and functional studies of Bud23-Trm112 reveal 18S rRNA N7-G1575 methylation occurs on late 40S precursor ribosomes.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 111, Page E5518-E5526, Year 2014
Publish dateJul 9, 2014 (structure data deposition date)
AuthorsLetoquart, J. / Huvelle, E. / Wacheul, L. / Bourgeois, G. / Zorbas, C. / Graille, M. / Heurgue-Hamard, V. / Lafontaine, D.L.
External linksProc. Natl. Acad. Sci. USA / PubMed:25489090
MethodsX-ray diffraction
Resolution2 - 2.124 Å
Structure data

PDB-4qtt:
Structure of S. cerevisiae Bud23-Trm112 complex involved in formation of m7G1575 on 18S rRNA (apo-form)
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4qtu:
Structure of S. cerevisiae Bud23-Trm112 complex involved in formation of m7G1575 on 18S rRNA (SAM bound form)
Method: X-RAY DIFFRACTION / Resolution: 2.124 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-12P:
DODECAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-SAM:
S-ADENOSYLMETHIONINE

Source
  • saccharomyces cerevisiae s288c (yeast)
KeywordsTRANSFERASE / Class I MTase / Methyltransferase / Methylation / Class I

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