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-Structure paper
タイトル | Semi-automated selection of cryo-EM particles in RELION-1.3. |
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ジャーナル・号・ページ | J Struct Biol, Vol. 189, Issue 2, Page 114-122, Year 2015 |
掲載日 | 2014年12月6日 |
著者 | Sjors H W Scheres / |
PubMed 要旨 | The selection of particles suitable for high-resolution cryo-EM structure determination from noisy micrographs may represent a tedious and time-consuming step. Here, a semi-automated particle ...The selection of particles suitable for high-resolution cryo-EM structure determination from noisy micrographs may represent a tedious and time-consuming step. Here, a semi-automated particle selection procedure is presented that has been implemented within the open-source software RELION. At the heart of the procedure lies a fully CTF-corrected template-based picking algorithm, which is supplemented by a fast sorting algorithm and reference-free 2D class averaging to remove false positives. With only limited user-interaction, the proposed procedure yields results that are comparable to manual particle selection. Together with an improved graphical user interface, these developments further contribute to turning RELION from a stand-alone refinement program into a convenient image processing pipeline for the entire single-particle approach. |
リンク | J Struct Biol / PubMed:25486611 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 4.2 Å |
構造データ | EMDB-2824: |
由来 |
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