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TitleSemi-automated selection of cryo-EM particles in RELION-1.3.
Journal, issue, pagesJ Struct Biol, Vol. 189, Issue 2, Page 114-122, Year 2015
Publish dateDec 6, 2014
AuthorsSjors H W Scheres /
PubMed AbstractThe selection of particles suitable for high-resolution cryo-EM structure determination from noisy micrographs may represent a tedious and time-consuming step. Here, a semi-automated particle ...The selection of particles suitable for high-resolution cryo-EM structure determination from noisy micrographs may represent a tedious and time-consuming step. Here, a semi-automated particle selection procedure is presented that has been implemented within the open-source software RELION. At the heart of the procedure lies a fully CTF-corrected template-based picking algorithm, which is supplemented by a fast sorting algorithm and reference-free 2D class averaging to remove false positives. With only limited user-interaction, the proposed procedure yields results that are comparable to manual particle selection. Together with an improved graphical user interface, these developments further contribute to turning RELION from a stand-alone refinement program into a convenient image processing pipeline for the entire single-particle approach.
External linksJ Struct Biol / PubMed:25486611 / PubMed Central
MethodsEM (single particle)
Resolution4.2 Å
Structure data

EMDB-2824:
Semi-automated selection of cryo-EM particles in RELION-1.3
Method: EM (single particle) / Resolution: 4.2 Å

Source
  • Escherichia coli K-12 (bacteria)

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