タイトル | Directed evolution of new and improved enzyme functions using an evolutionary intermediate and multidirectional search. |
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ジャーナル・号・ページ | Acs Chem. Biol., Vol. 10, Page 611-621, Year 2015 |
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掲載日 | 2014年7月16日 (構造データの登録日) |
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著者 | Porter, J.L. / Boon, P.L. / Murray, T.P. / Huber, T. / Collyer, C.A. / Ollis, D.L. |
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リンク | Acs Chem. Biol. / PubMed:25419863 |
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手法 | X線回折 |
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解像度 | 1.67 - 1.95 Å |
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構造データ | PDB-4u2b: Crystal structure of dienelactone hydrolase (C123S) at 1.70 A resolution 手法: X-RAY DIFFRACTION / 解像度: 1.7 Å PDB-4u2c: Crystal structure of dienelactone hydrolase A-6 variant (S7T, A24V, Q35H, F38L, Q110L, C123S, Y145C, E199G and S208G) at 1.95 A resolution 手法: X-RAY DIFFRACTION / 解像度: 1.95 Å PDB-4u2d: Crystal structure of dienelactone hydrolase S-2 variant (Q35H, F38L, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G and G211D) at 1.67 A resolution 手法: X-RAY DIFFRACTION / 解像度: 1.67 Å PDB-4u2e: Crystal structure of dienelactone hydrolase S-3 variant (Q35H, F38L, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G, G211D and K234N) at 1.70 A resolution 手法: X-RAY DIFFRACTION / 解像度: 1.7 Å PDB-4u2f: Crystal structure of dienelactone hydrolase B-1 variant (Q35H, F38L, Y64H, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G and G211D) at 1.80 A resolution 手法: X-RAY DIFFRACTION / 解像度: 1.8 Å PDB-4u2g: Crystal structure of dienelactone hydrolase B-4 variant (Q35H, F38L, Y64H, Q76L, Q110L, C123S, Y137C, A141V, Y145C, N154D, E199G, S208G, G211D, S233G and 237Q) at 1.80 A resolution 手法: X-RAY DIFFRACTION / 解像度: 1.8 Å |
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化合物 | |
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由来 | - pseudomonas knackmussii (バクテリア)
- pseudomonas sp. (バクテリア)
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キーワード | HYDROLASE / a/b hydrolase fold |
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