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Structure paper

TitleCholesterol ester oxidation by mycobacterial cytochrome p450.
Journal, issue, pagesJ. Biol. Chem., Vol. 289, Page 30417-30425, Year 2014
Publish dateJun 17, 2014 (structure data deposition date)
AuthorsFrank, D.J. / Madrona, Y. / Ortiz de Montellano, P.R.
External linksJ. Biol. Chem. / PubMed:25210044
MethodsX-ray diffraction
Resolution1.78 - 2 Å
Structure data

PDB-4tri:
X-ray crystal structure of CYP142A2 from Mycobacterium smegmatis, complexed with cholesterol sulfate.
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4uax:
X-ray crystal structure of ligand free CYP142A2 from Mycobacterium smegmatis
Method: X-RAY DIFFRACTION / Resolution: 1.78 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-C3S:
CHOLEST-5-EN-3-YL HYDROGEN SULFATE

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • mycobacterium smegmatis (bacteria)
KeywordsOXIDOREDUCTASE / Cytochrome P450 / cholesterol sulfate / ligand bound / hemoprotein

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