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Structure paper

TitleHuman oxygen sensing may have origins in prokaryotic elongation factor Tu prolyl-hydroxylation
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 111, Page 13331-13336, Year 2014
Publish dateJan 23, 2013 (structure data deposition date)
AuthorsScotti, J.S. / Leung, I.K.H. / Ge, W. / Bentley, M.A. / Paps, J. / Kramer, H.B. / Lee, J. / Aik, W. / Choi, H. / Paulsen, S.M. ...Scotti, J.S. / Leung, I.K.H. / Ge, W. / Bentley, M.A. / Paps, J. / Kramer, H.B. / Lee, J. / Aik, W. / Choi, H. / Paulsen, S.M. / Bowman, L.A.H. / Loik, N.D. / Horita, S. / Ho, C.H. / Kershaw, N.J. / Tang, C.M. / Claridge, T.D.W. / Preston, G.M. / McDonough, M.A. / Schofield, C.J.
External linksProc. Natl. Acad. Sci. USA / PubMed:25197067
MethodsX-ray diffraction
Resolution1.97 - 2.697 Å
Structure data

PDB-4iw3:
Crystal structure of a Pseudomonas putida prolyl-4-hydroxylase (P4H) in complex with elongation factor Tu (EF-Tu)
Method: X-RAY DIFFRACTION / Resolution: 2.697 Å

PDB-4j0q:
Crystal structure of Pseudomonas putida elongation factor Tu (EF-Tu)
Method: X-RAY DIFFRACTION / Resolution: 2.294 Å

PDB-4j25:
Crystal structure of a Pseudomonas putida prolyl-4-hydroxylase (P4H)
Method: X-RAY DIFFRACTION / Resolution: 1.97 Å

Chemicals

ChemComp-MN:
Unknown entry

ChemComp-OGA:
N-OXALYLGLYCINE / inhibitor*YM

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

Source
  • pseudomonas putida (bacteria)
KeywordsMETAL BINDING PROTEIN/TRANSLATION / 2-oxoglutarate oxygenase / oxygen sensing / protein synthesis regulation / double-stranded beta helix / jellyroll fold / prolyl-4-hydroxylase / translation / METAL BINDING PROTEIN-TRANSLATION complex / GDP / GTP / GTPase / elongation / pseudomonas / OXIDOREDUCTASE

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