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TitleSpatial localization of the Ebola virus glycoprotein mucin-like domain determined by cryo-electron tomography.
Journal, issue, pagesJ Virol, Vol. 88, Issue 18, Page 10958-10962, Year 2014
Publish dateJul 9, 2014
AuthorsErin E H Tran / James A Simmons / Alberto Bartesaghi / Charles J Shoemaker / Elizabeth Nelson / Judith M White / Sriram Subramaniam /
PubMed AbstractThe Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three- ...The Ebola virus glycoprotein mucin-like domain (MLD) is implicated in Ebola virus cell entry and immune evasion. Using cryo-electron tomography of Ebola virus-like particles, we determined a three-dimensional structure for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein lacking the MLD. Our results, which show that the MLD is located at the apex and the sides of each glycoprotein monomer, provide a structural template for analysis of MLD function.
External linksJ Virol / PubMed:25008940 / PubMed Central
MethodsEM (subtomogram averaging)
Structure data

EMDB-6003:
Cryo-electron tomography of full-length glycoprotein from Ebola virus-like particles
Method: EM (subtomogram averaging)

EMDB-6004:
Cryo-electron tomography of glycoprotein lacking the mucin-like domain from Ebola virus-like particles
Method: EM (subtomogram averaging)

Source
  • Zaire ebolavirus

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