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Structure paper

TitleStructural basis for glucose tolerance in GH1 beta-glucosidases.
Journal, issue, pagesActa Crystallogr. ,Sect. D, Vol. 70, Page 1631-1639, Year 2014
Publish dateAug 23, 2013 (structure data deposition date)
AuthorsGiuseppe, P.O. / Souza, T.A.C.B. / Souza, F.H.M. / Zanphorlin, L.M. / Machado, C.B. / Ward, R.J. / Jorge, J.A. / Furriel, R.P.M. / Murakami, M.T.
External linksActa Crystallogr. ,Sect. D / PubMed:24914974
MethodsX-ray diffraction
Resolution2.05 - 2.6 Å
Structure data

PDB-4mdo:
Crystal structure of a GH1 beta-glucosidase from the fungus Humicola insolens
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-4mdp:
Crystal structure of a GH1 beta-glucosidase from the fungus Humicola insolens in complex with glucose
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

Chemicals

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-BGC:
beta-D-glucopyranose

ChemComp-PO4:
PHOSPHATE ION

ChemComp-GOL:
GLYCEROL

Source
  • humicola grisea var. thermoidea (fungus)
KeywordsHYDROLASE / Glycoside Hydrolase Family 1 / TIM barrel / retaining enzyme

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