EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	New insights into rotavirus entry machinery: stabilization of rotavirus spike conformation is independent of trypsin cleavage.
ジャーナル・号・ページ	PLoS Pathog, Vol. 10, Issue 5, Page e1004157, Year 2014
掲載日	2014年5月29日
著者	Javier M Rodríguez / Francisco J Chichón / Esther Martín-Forero / Fernando González-Camacho / José L Carrascosa / José R Castón / Daniel Luque /
PubMed 要旨	The infectivity of rotavirus, the main causative agent of childhood diarrhea, is dependent on activation of the extracellular viral particles by trypsin-like proteases in the host intestinal lumen. ...The infectivity of rotavirus, the main causative agent of childhood diarrhea, is dependent on activation of the extracellular viral particles by trypsin-like proteases in the host intestinal lumen. This step entails proteolytic cleavage of the VP4 spike protein into its mature products, VP8* and VP5*. Previous cryo-electron microscopy (cryo-EM) analysis of trypsin-activated particles showed well-resolved spikes, although no density was identified for the spikes in uncleaved particles; these data suggested that trypsin activation triggers important conformational changes that give rise to the rigid, entry-competent spike. The nature of these structural changes is not well understood, due to lack of data relative to the uncleaved spike structure. Here we used cryo-EM and cryo-electron tomography (cryo-ET) to characterize the structure of the uncleaved virion in two model rotavirus strains. Cryo-EM three-dimensional reconstruction of uncleaved virions showed spikes with a structure compatible with the atomic model of the cleaved spike, and indistinguishable from that of digested particles. Cryo-ET and subvolume average, combined with classification methods, resolved the presence of non-icosahedral structures, providing a model for the complete structure of the uncleaved spike. Despite the similar rigid structure observed for uncleaved and cleaved particles, trypsin activation is necessary for successful infection. These observations suggest that the spike precursor protein must be proteolytically processed, not to achieve a rigid conformation, but to allow the conformational changes that drive virus entry.
リンク	PLoS Pathog / PubMed:24873828 / PubMed Central
手法	EM (単粒子) / EM (サブトモグラム平均)
解像度	14.5 - 17.4 Å
構造データ	EMDB-2573: SA11 Rotavirus non-trypsinized Triple Layered Particle 手法: EM (単粒子) / 解像度: 15.4 Å EMDB-2574: SA11 Rotavirus trypsinized Triple Layered Particle 手法: EM (単粒子) / 解像度: 14.5 Å EMDB-2575: SA11 Rotavirus non-trypsinized Triple Layered Particle grown in absence of leupetin 手法: EM (単粒子) / 解像度: 15.9 Å EMDB-2576: OSU Rotavirus non-trypsinized Triple Layered Particle 手法: EM (単粒子) / 解像度: 16.2 Å EMDB-2577: OSU Rotavirus trypsinized Triple Layered Particle 手法: EM (単粒子) / 解像度: 17.4 Å EMDB-2578: Class 1 averaged spike from SA11 Rotavirus non-trypsinized Triple Layered Particle 手法: EM (サブトモグラム平均) EMDB-2579: Class 2 averaged spike from SA11 Rotavirus non-trypsinized Triple Layered Particle 手法: EM (サブトモグラム平均) EMDB-2580: Class 1 averaged spike from SA11 Rotavirus trypsinized Triple Layered Particle 手法: EM (サブトモグラム平均)