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Structure paper

TitleDetermination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL.
Journal, issue, pagesNat. Methods, Vol. 11, Page 734-736, Year 2014
Publish dateJul 29, 2013 (structure data deposition date)
AuthorsHirata, K. / Shinzawa-Itoh, K. / Yano, N. / Takemura, S. / Kato, K. / Hatanaka, M. / Muramoto, K. / Kawahara, T. / Tsukihara, T. / Yamashita, E. ...Hirata, K. / Shinzawa-Itoh, K. / Yano, N. / Takemura, S. / Kato, K. / Hatanaka, M. / Muramoto, K. / Kawahara, T. / Tsukihara, T. / Yamashita, E. / Tono, K. / Ueno, G. / Hikima, T. / Murakami, H. / Inubushi, Y. / Yabashi, M. / Ishikawa, T. / Yamamoto, M. / Ogura, T. / Sugimoto, H. / Shen, J.R. / Yoshikawa, S. / Ago, H.
External linksNat. Methods / PubMed:24813624
MethodsX-ray diffraction
Resolution1.9 Å
Structure data

PDB-3wg7:
A 1.9 angstrom radiation damage free X-ray structure of large (420KDa) protein by femtosecond crystallography
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-7y44:
Re-refinement of damage free X-ray structure of bovine cytochrome c oxidase at 1.9 angstrom resolution
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-HEA:
HEME-A

ChemComp-CU:
COPPER (II) ION

ChemComp-MG:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-PER:
PEROXIDE ION

ChemComp-PGV:
(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE / phospholipid*YM

ChemComp-TGL:
TRISTEAROYLGLYCEROL

ChemComp-CUA:
DINUCLEAR COPPER ION

ChemComp-CHD:
CHOLIC ACID

ChemComp-PSC:
(7R,17E,20E)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSA-17,20-DIEN-1-AMINIUM 4-OXIDE / phospholipid*YM

ChemComp-PEK:
(1S)-2-{[(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(STEAROYLOXY)METHYL]ETHYL (5E,8E,11E,14E)-ICOSA-5,8,11,14-TETRAENOATE / phospholipid*YM

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

ChemComp-ZN:
Unknown entry

ChemComp-DMU:
DECYL-BETA-D-MALTOPYRANOSIDE / detergent*YM

ChemComp-HOH:
WATER

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PO4:
PHOSPHATE ION

Source
  • bos taurus (domestic cattle)
KeywordsOXIDOREDUCTASE / Electron Transport Complex IV / Iron / Oxidation-Reduction / Protein Interaction Domains / Motifs / Membrane / respiratory enzyme / membrane protein / heme protein

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