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-Structure paper
タイトル | Initiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome. |
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ジャーナル・号・ページ | Cell, Vol. 157, Issue 4, Page 823-831, Year 2014 |
掲載日 | 2014年5月8日 |
著者 | Israel S Fernández / Xiao-Chen Bai / Garib Murshudov / Sjors H W Scheres / V Ramakrishnan / |
PubMed 要旨 | The cricket paralysis virus internal ribosome entry site (CrPV-IRES) is a folded structure in a viral mRNA that allows initiation of translation in the absence of any host initiation factors. By ...The cricket paralysis virus internal ribosome entry site (CrPV-IRES) is a folded structure in a viral mRNA that allows initiation of translation in the absence of any host initiation factors. By using recent advances in single-particle electron cryomicroscopy, we have solved the structure of CrPV-IRES bound to the ribosome of the yeast Kluyveromyces lactis in both the canonical and rotated states at overall resolutions of 3.7 and 3.8 Å, respectively. In both states, the pseudoknot PKI of the CrPV-IRES mimics a tRNA/mRNA interaction in the decoding center of the A site of the 40S ribosomal subunit. The structure and accompanying factor-binding data show that CrPV-IRES binding mimics a pretranslocation rather than initiation state of the ribosome. Translocation of the IRES by elongation factor 2 (eEF2) is required to bring the first codon of the mRNA into the A site and to allow the start of translation. |
リンク | Cell / PubMed:24792965 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.6 - 3.8 Å |
構造データ | EMDB-2599, PDB-4v91: |
由来 |
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キーワード | RIBOSOME / TRANSLATION / INITIATION / IRES / EUKARYOTIC |