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TitleInitiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome.
Journal, issue, pagesCell, Vol. 157, Issue 4, Page 823-831, Year 2014
Publish dateMay 8, 2014
AuthorsIsrael S Fernández / Xiao-Chen Bai / Garib Murshudov / Sjors H W Scheres / V Ramakrishnan /
PubMed AbstractThe cricket paralysis virus internal ribosome entry site (CrPV-IRES) is a folded structure in a viral mRNA that allows initiation of translation in the absence of any host initiation factors. By ...The cricket paralysis virus internal ribosome entry site (CrPV-IRES) is a folded structure in a viral mRNA that allows initiation of translation in the absence of any host initiation factors. By using recent advances in single-particle electron cryomicroscopy, we have solved the structure of CrPV-IRES bound to the ribosome of the yeast Kluyveromyces lactis in both the canonical and rotated states at overall resolutions of 3.7 and 3.8 Å, respectively. In both states, the pseudoknot PKI of the CrPV-IRES mimics a tRNA/mRNA interaction in the decoding center of the A site of the 40S ribosomal subunit. The structure and accompanying factor-binding data show that CrPV-IRES binding mimics a pretranslocation rather than initiation state of the ribosome. Translocation of the IRES by elongation factor 2 (eEF2) is required to bring the first codon of the mRNA into the A site and to allow the start of translation.
External linksCell / PubMed:24792965 / PubMed Central
MethodsEM (single particle)
Resolution3.6 - 3.8 Å
Structure data

2599

4v91

EMDB-2599, PDB-4v91:
Kluyveromyces lactis 80S ribosome in complex with CrPV-IRES
Method: EM (single particle) / Resolution: 3.7 Å

2603

4v92

EMDB-2603, PDB-4v92:
Kluyveromyces lactis 80S ribosome in complex with CrPV-IRES
Method: EM (single particle) / Resolution: 3.6 Å

2604

4v92

EMDB-2604: Kluyveromyces lactis 80S ribosome in complex with CrPV-IRES in a rotated stated with masked 40S
PDB-4v92: Kluyveromyces lactis 80S ribosome in complex with CrPV-IRES
Method: EM (single particle) / Resolution: 3.8 Å

Source
  • kluyveromyces lactis (yeast)
  • cricket paralysis virus
KeywordsRIBOSOME / TRANSLATION / INITIATION / IRES / EUKARYOTIC

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