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TitleThe role of distant mutations and allosteric regulation on LovD active site dynamics.
Journal, issue, pagesNat. Chem. Biol., Vol. 10, Page 431-436, Year 2014
Publish dateJun 21, 2013 (structure data deposition date)
AuthorsJimenez-Oses, G. / Osuna, S. / Gao, X. / Sawaya, M.R. / Gilson, L. / Collier, S.J. / Huisman, G.W. / Yeates, T.O. / Tang, Y. / Houk, K.N.
External linksNat. Chem. Biol. / PubMed:24727900
MethodsX-ray diffraction
Resolution1.8 - 3.19 Å
Structure data

PDB-4lcl:
Simvastatin Synthase (LOVD), from Aspergillus Terreus, LovD6 mutant (simh6208)
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-4lcm:
Simvastatin Synthase (LOVD), from Aspergillus Terreus, LovD9 mutant (simh9014)
Method: X-RAY DIFFRACTION / Resolution: 3.19 Å

Chemicals

ChemComp-IPA:
ISOPROPYL ALCOHOL / alkaloid*YM

ChemComp-HOH:
WATER

Source
  • aspergillus terreus (mold)
KeywordsTRANSFERASE / laboratory-directed evolution / Transesterase

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