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Structure paper

TitleStructure-guided mutation of the conserved G3-box glycine in Rheb generates a constitutively activated regulator of mammalian target of rapamycin (mTOR).
Journal, issue, pagesJ. Biol. Chem., Vol. 289, Page 12195-12201, Year 2014
Publish dateDec 16, 2013 (structure data deposition date)
AuthorsMazhab-Jafari, M.T. / Marshall, C.B. / Ho, J. / Ishiyama, N. / Stambolic, V. / Ikura, M.
External linksJ. Biol. Chem. / PubMed:24648513
MethodsX-ray diffraction
Resolution2.2 - 2.4 Å
Structure data

PDB-4o25:
Structure of Wild Type Mus musculus Rheb bound to GTP
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-4o2l:
Structure of Mus musculus Rheb G63A mutant bound to GTP
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-4o2r:
Structure of Mus musculus Rheb G63V mutant bound to GDP
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-ACT:
ACETATE ION

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Source
  • mus musculus (house mouse)
KeywordsHYDROLASE / small GTPase / GTP and GDP / GDP and GTP / GDP

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