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TitleVisualization of two transfer RNAs trapped in transit during elongation factor G-mediated translocation.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 110, Issue 52, Page 20964-20969, Year 2013
Publish dateDec 24, 2013
AuthorsDavid J F Ramrath / Laura Lancaster / Thiemo Sprink / Thorsten Mielke / Justus Loerke / Harry F Noller / Christian M T Spahn /
PubMed AbstractDuring protein synthesis, coupled translocation of messenger RNAs (mRNA) and transfer RNAs (tRNA) through the ribosome takes place following formation of each peptide bond. The reaction is ...During protein synthesis, coupled translocation of messenger RNAs (mRNA) and transfer RNAs (tRNA) through the ribosome takes place following formation of each peptide bond. The reaction is facilitated by large-scale conformational changes within the ribosomal complex and catalyzed by elongtion factor G (EF-G). Previous structural analysis of the interaction of EF-G with the ribosome used either model complexes containing no tRNA or only a single tRNA, or complexes where EF-G was directly bound to ribosomes in the posttranslocational state. Here, we present a multiparticle cryo-EM reconstruction of a translocation intermediate containing two tRNAs trapped in transit, bound in chimeric intrasubunit ap/P and pe/E hybrid states. The downstream ap/P-tRNA is contacted by domain IV of EF-G and P-site elements within the 30S subunit body, whereas the upstream pe/E-tRNA maintains tight interactions with P-site elements of the swiveled 30S head. Remarkably, a tight compaction of the tRNA pair can be seen in this state. The translocational intermediate presented here represents a previously missing link in understanding the mechanism of translocation, revealing that the ribosome uses two distinct molecular ratchets, involving both intra- and intersubunit rotational movements, to drive the synchronous movement of tRNAs and mRNA.
External linksProc Natl Acad Sci U S A / PubMed:24324168 / PubMed Central
MethodsEM (single particle)
Resolution6.8 Å
Structure data

5775

4v7b

EMDB-5775: Cryo-EM reconstruction of a authentic 70S-tRNA2-mRNA-EF-G translocation intermediate
PDB-4v7b: Visualization of two tRNAs trapped in transit during EF-G-mediated translocation
Method: EM (single particle) / Resolution: 6.8 Å

Chemicals

ChemComp-FUA:
FUSIDIC ACID / antibiotic, Antimicrobial*YM / Fusidic acid

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

Source
  • escherichia coli (E. coli)
KeywordsRIBOSOME/TRANSLATION / translocation intermediate / EF-G / head swiveling / RIBOSOME-TRANSLATION complex

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