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Structure paper

TitleCrystal structure of tRNA m1G9 methyltransferase Trm10: insight into the catalytic mechanism and recognition of tRNA substrate.
Journal, issue, pagesNucleic Acids Res., Vol. 42, Page 509-525, Year 2014
Publish dateMar 27, 2013 (structure data deposition date)
AuthorsShao, Z. / Yan, W. / Peng, J. / Zuo, X. / Zou, Y. / Li, F. / Gong, D. / Ma, R. / Wu, J. / Shi, Y. ...Shao, Z. / Yan, W. / Peng, J. / Zuo, X. / Zou, Y. / Li, F. / Gong, D. / Ma, R. / Wu, J. / Shi, Y. / Zhang, Z. / Teng, M. / Li, X. / Gong, Q.
External linksNucleic Acids Res. / PubMed:24081582
MethodsX-ray diffraction
Resolution1.76 - 2.5 Å
Structure data

PDB-4jwf:
Crystal structure of spTrm10(74)-SAH complex
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-4jwg:
Crystal structure of spTrm10(74)
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-4jwh:
Crystal structure of spTrm10(Full length)-SAH complex
Method: X-RAY DIFFRACTION / Resolution: 2.04 Å

PDB-4jwj:
Crystal structure of scTrm10(84)-SAH complex
Method: X-RAY DIFFRACTION / Resolution: 1.76 Å

Chemicals

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-ACY:
ACETIC ACID

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

Source
  • schizosaccharomyces pombe (fission yeast)
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSFERASE / tRNA MTase domain

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