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TitleCytochrome p450sky interacts directly with the nonribosomal Peptide synthetase to generate three amino Acid precursors in skyllamycin biosynthesis.
Journal, issue, pagesAcs Chem. Biol., Vol. 8, Page 2586-2596, Year 2013
Publish dateMay 31, 2013 (structure data deposition date)
AuthorsUhlmann, S. / Sussmuth, R.D. / Cryle, M.J.
External linksAcs Chem. Biol. / PubMed:24079328
MethodsX-ray diffraction
Resolution1.9 - 2.7 Å
Structure data

PDB-4l0e:
Structure of P450sky (CYP163B3), a cytochrome P450 from skyllamycin biosynthesis (heme-coordinated expression tag)
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-4l0f:
Structure of P450sky (CYP163B3), a cytochrome P450 from skyllamycin biosynthesis (open active site)
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PGE:
TRIETHYLENE GLYCOL

Source
  • streptomyces sp. (bacteria)
KeywordsOXIDOREDUCTASE / Cytochrome P450 / Skyllamycin biosynthesis / Beta-aminoacyl carrier protein oxidase / Skyllamycin nonribosomal peptide synthetase

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