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TitleMaximizing the potential of electron cryomicroscopy data collected using direct detectors.
Journal, issue, pagesJ Struct Biol, Vol. 184, Issue 2, Page 193-202, Year 2013
Publish dateSep 12, 2013
AuthorsDavid Veesler / Melody G Campbell / Anchi Cheng / Chi-Yu Fu / Zachary Murez / John E Johnson / Clinton S Potter / Bridget Carragher /
PubMed AbstractSingle-particle electron cryomicroscopy is undergoing a technical revolution due to the recent developments of direct detectors. These new recording devices detect electrons directly (i.e. without ...Single-particle electron cryomicroscopy is undergoing a technical revolution due to the recent developments of direct detectors. These new recording devices detect electrons directly (i.e. without conversion into light) and feature significantly improved detective quantum efficiencies and readout rates as compared to photographic films or CCDs. We evaluated here the potential of one such detector (Gatan K2 Summit) to enable the achievement of near-atomic resolution reconstructions of biological specimens when coupled to a widely used, mid-range transmission electron microscope (FEI TF20 Twin). Compensating for beam-induced motion and stage drift provided a 4.4Å resolution map of Sulfolobus turreted icosahedral virus (STIV), which we used as a test particle in this study. Several motion correction and dose fractionation procedures were explored and we describe their influence on the resolution of the final reconstruction. We also compared the quality of this data to that collected with a FEI Titan Krios microscope equipped with a Falcon I direct detector, which provides a benchmark for data collected using a high-end electron microscope.
External linksJ Struct Biol / PubMed:24036281 / PubMed Central
MethodsEM (single particle)
Resolution5.8 Å
Structure data

EMDB-5754:
Maximizing the potential of electron cryomicroscopy data collected using direct detectors
Method: EM (single particle) / Resolution: 5.8 Å

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