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-Structure paper
タイトル | The structure and host entry of an invertebrate parvovirus. |
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ジャーナル・号・ページ | J Virol, Vol. 87, Issue 23, Page 12523-12530, Year 2013 |
掲載日 | 2013年9月11日 |
![]() | Geng Meng / Xinzheng Zhang / Pavel Plevka / Qian Yu / Peter Tijssen / Michael G Rossmann / ![]() |
PubMed 要旨 | The 3.5-Å resolution X-ray crystal structure of mature cricket parvovirus (Acheta domesticus densovirus [AdDNV]) has been determined. Structural comparisons show that vertebrate and invertebrate ...The 3.5-Å resolution X-ray crystal structure of mature cricket parvovirus (Acheta domesticus densovirus [AdDNV]) has been determined. Structural comparisons show that vertebrate and invertebrate parvoviruses have evolved independently, although there are common structural features among all parvovirus capsid proteins. It was shown that raising the temperature of the AdDNV particles caused a loss of their genomes. The structure of these emptied particles was determined by cryo-electron microscopy to 5.5-Å resolution, and the capsid structure was found to be the same as that for the full, mature virus except for the absence of the three ordered nucleotides observed in the crystal structure. The viral protein 1 (VP1) amino termini could be externalized without significant damage to the capsid. In vitro, this externalization of the VP1 amino termini is accompanied by the release of the viral genome. |
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手法 | EM (単粒子) / X線回折 |
解像度 | 3.5 Å |
構造データ | ![]() EMDB-2401: ![]() PDB-4mgu: |
由来 |
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![]() | VIRUS/DNA / Virus / Jelly-Roll / DNV / VIRUS-DNA complex |