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Structure paper

TitleGeneral approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 110, Page 10946-10951, Year 2013
Publish dateMay 15, 2013 (structure data deposition date)
AuthorsBrinkmann-Chen, S. / Flock, T. / Cahn, J.K. / Snow, C.D. / Brustad, E.M. / McIntosh, J.A. / Meinhold, P. / Zhang, L. / Arnold, F.H.
External linksProc. Natl. Acad. Sci. USA / PubMed:23776225
MethodsX-ray diffraction
Resolution1.39 - 1.8 Å
Structure data

PDB-4kqw:
The structure of the Slackia exigua KARI in complex with NADP
Method: X-RAY DIFFRACTION / Resolution: 1.39 Å

PDB-4kqx:
Mutant Slackia exigua KARI DDV in complex with NAD and an inhibitor
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-TLA:
L(+)-TARTARIC ACID

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-HIO:
N-HYDROXY-N-ISOPROPYLOXAMIC ACID

ChemComp-HIS:
HISTIDINE

Source
  • slackia exigua (bacteria)
KeywordsOXIDOREDUCTASE / Rossmann Fold / Ketol-acid reductoisomerase / acetohydroxyacid isomeroreductase / OXIDOREDUCTASE/OXIDOREDUCTASE INHIBITOR / cofactor switch / OXIDOREDUCTASE-OXIDOREDUCTASE INHIBITOR complex

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