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Structure paper

TitleA ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression.
Journal, issue, pagesNat. Struct. Mol. Biol., Vol. 20, Page 859-867, Year 2013
Publish dateOct 9, 2012 (structure data deposition date)
AuthorsWinkler, A. / Heintz, U. / Lindner, R. / Reinstein, J. / Shoeman, R.L. / Schlichting, I.
External linksNat. Struct. Mol. Biol. / PubMed:23728293
MethodsX-ray diffraction
Resolution1.75 - 3.501 Å
Structure data

PDB-4hh0:
Dark-state structure of AppA C20S without the Cys-rich region from Rb. sphaeroides
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-4hh1:
Dark-state structure of AppA wild-type without the Cys-rich region from Rb. sphaeroides
Method: X-RAY DIFFRACTION / Resolution: 3.501 Å

PDB-4hh2:
Structure of PpsR without the HTH motif from Rb. sphaeroides
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-4hh3:
Structure of the AppA-PpsR2 core complex from Rb. sphaeroides
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

Chemicals

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

Source
  • rhodobacter sphaeroides (bacteria)
KeywordsFLAVOPROTEIN / SIGNALING PROTEIN / BLUF domain / SCHIC domain / photoreceptor / PpsR / TRANSCRIPTION / triple PAS domain / Q-linker / DNA-binding / AppA / FLAVOPROTEIN/TRANSCRIPTION / helix bundle / PAS domain / AppA-PpsR complex / FLAVOPROTEIN-TRANSCRIPTION complex

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