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Structure paper

TitleCyclic [G(2',5')pA(3',5')p] is the metazoan second messenger produced by DNA-activated cyclic GMP-AMP synthase.
Journal, issue, pagesCell(Cambridge,Mass. ), Vol. 153, Page 1094-1107, Year 2013
Publish dateApr 18, 2013 (structure data deposition date)
AuthorsGao, P. / Ascano, M. / Wu, Y. / Barchet, W. / Gaffney, B.L. / Zillinger, T. / Serganov, A.A. / Liu, Y. / Jones, R.A. / Hartmann, G. ...Gao, P. / Ascano, M. / Wu, Y. / Barchet, W. / Gaffney, B.L. / Zillinger, T. / Serganov, A.A. / Liu, Y. / Jones, R.A. / Hartmann, G. / Tuschl, T. / Patel, D.J.
External linksCell(Cambridge,Mass. ) / PubMed:23647843
MethodsX-ray diffraction
Resolution1.94 - 2.41 Å
Structure data

PDB-4k8v:
Structure of cyclic GMP-AMP Synthase (cGAS)
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4k96:
Structure of Binary Complex of cGAS with Bound dsDNA
Method: X-RAY DIFFRACTION / Resolution: 2.084 Å

PDB-4k97:
Structure of Ternary Complex of cGAS with dsDNA and Bound ATP
Method: X-RAY DIFFRACTION / Resolution: 2.41 Å

PDB-4k98:
Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pppG(2 ,5 )pG
Method: X-RAY DIFFRACTION / Resolution: 1.94 Å

PDB-4k99:
Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pppdG(2 ,5 )pdG
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-4k9a:
Structure of Ternary Complex of cGAS with dsDNA and Bound 5 -pG(2 ,5 )pA
Method: X-RAY DIFFRACTION / Resolution: 2.264 Å

PDB-4k9b:
Structure of Ternary Complex of cGAS with dsDNA and Bound c[G(2 ,5 )pA(3 ,5 )p]
Method: X-RAY DIFFRACTION / Resolution: 2.26 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-5GP:
GUANOSINE-5'-MONOPHOSPHATE

ChemComp-GH3:
3'-DEOXY-GUANOSINE-5'-TRIPHOSPHATE

ChemComp-GDO:
3'-deoxy-guanosine 5'-monophosphate

ChemComp-PO4:
PHOSPHATE ION

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM

Source
  • mus musculus (house mouse)
  • synthetic construct (others)
KeywordsTRANSFERASE / nucleotidyltransferase / DNA / TRANSFERASE/DNA / nucleotidyltransferase fold / TRANSFERASE-DNA complex

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