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Structure paper

TitleAmino Acid Substitution in the Active Site of DNA Polymerase beta Explains the Energy Barrier of the Nucleotidyl Transfer Reaction.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 135, Page 8078-8088, Year 2013
Publish dateMar 27, 2013 (structure data deposition date)
AuthorsBatra, V.K. / Perera, L. / Lin, P. / Shock, D.D. / Beard, W.A. / Pedersen, L.C. / Pedersen, L.G. / Wilson, S.H.
External linksJ. Am. Chem. Soc. / PubMed:23647366
MethodsX-ray diffraction
Resolution2 - 2.39 Å
Structure data

PDB-4jwm:
Ternary complex of D256E mutant of DNA Polymerase Beta
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4jwn:
Ternary complex of D256A mutant of DNA Polymerase Beta
Method: X-RAY DIFFRACTION / Resolution: 2.39 Å

Chemicals

ChemComp-DUP:
2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE

ChemComp-MG:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsTRANSFERASE/DNA / Nucleotidyl Transfer / dNTP / Non-hydrolyzable analog / TRANSFERASE-DNA complex

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