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Structure paper

TitleDifferent Photochemical Events of a Genetically Encoded Phenyl Azide Define and Modulate GFP Fluorescence.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 52, Page 5974-5977, Year 2013
Publish dateFeb 14, 2013 (structure data deposition date)
AuthorsReddington, S.C. / Rizkallah, P.J. / Watson, P.D. / Pearson, R. / Tippmann, E.M. / Jones, D.D.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:23620472
MethodsX-ray diffraction
Resolution1.26 - 2.1 Å
Structure data

PDB-4j88:
Dark-state structure of sfGFP containing the unnatural amino acid p-azido-phenylalanine at residue 66
Method: X-RAY DIFFRACTION / Resolution: 2.08 Å

PDB-4j89:
Different photochemical events of a genetically encoded aryl azide define and modulate GFP fluorescence
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-4j8a:
Irradiated-state structure of sfGFP containing the unnatural amino acid p-azido-phenylalanine at residue 145
Method: X-RAY DIFFRACTION / Resolution: 1.26 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-HOH:
WATER

Source
  • aequorea victoria (jellyfish)
KeywordsFLUORESCENT PROTEIN / beta-barrel / Chromophore by cyclisation / p-Azido-L-Phenylalanine / cytosol

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