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Structure paper

TitleX-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 110, Page 7188-7192, Year 2013
Publish dateMar 28, 2013 (structure data deposition date)
AuthorsNicolet, Y. / Rohac, R. / Martin, L. / Fontecilla-Camps, J.C.
External linksProc. Natl. Acad. Sci. USA / PubMed:23596207
MethodsX-ray diffraction
Resolution1.45 - 2.901 Å
Structure data

PDB-4jxc:
X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-4jy8:
X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters
Method: X-RAY DIFFRACTION / Resolution: 2.901 Å

PDB-4jy9:
X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-4jyd:
X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters.
Method: X-RAY DIFFRACTION / Resolution: 1.71 Å

PDB-4jye:
X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters.
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-4jyf:
X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters.
Method: X-RAY DIFFRACTION / Resolution: 1.45 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-SAM:
S-ADENOSYLMETHIONINE

ChemComp-1N7:
CHAPSO / detergent*YM

ChemComp-GOL:
GLYCEROL

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

ChemComp-H2S:
HYDROSULFURIC ACID

ChemComp-IPA:
ISOPROPYL ALCOHOL

ChemComp-CL:
Unknown entry

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-CPS:
3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE / detergent*YM

ChemComp-FE:
Unknown entry

ChemComp-BR:
BROMIDE ION

ChemComp-F3S:
FE3-S4 CLUSTER

ChemComp-CO3:
CARBONATE ION

Source
  • thermotoga maritima (bacteria)
KeywordsOXIDOREDUCTASE / Radical SAM Protein / FeFe-hydrogenase active site Assembly / Fe4S4 cluster / S-adenosyl-L-methionine / Radical SAM enzyme / Assembly of the active site of FeFe-hydrogenases / Fe4S4 cluster S-adenosyl-L-methionine

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