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| Title | Cryo-EM model validation using independent map reconstructions. |
|---|---|
| Journal, issue, pages | Protein Sci, Vol. 22, Issue 6, Page 865-868, Year 2013 |
| Publish date | Dec 9, 2013 |
 Authors | Frank DiMaio / Junjie Zhang / Wah Chiu / David Baker /  |
| PubMed Abstract | An increasing number of cryo-electron microscopy (cryo-EM) density maps are being generated with suitable resolution to trace the protein backbone and guide sidechain placement. Generating and ...An increasing number of cryo-electron microscopy (cryo-EM) density maps are being generated with suitable resolution to trace the protein backbone and guide sidechain placement. Generating and evaluating atomic models based on such maps would be greatly facilitated by independent validation metrics for assessing the fit of the models to the data. We describe such a metric based on the fit of atomic models with independent test maps from single particle reconstructions not used in model refinement. The metric provides a means to determine the proper balance between the fit to the density and model energy and stereochemistry during refinement, and is likely to be useful in determining values of model building and refinement metaparameters quite generally. |
 External links | Protein Sci / PubMed:23592445 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 4.3 - 4.7 Å |
| Structure data | EMDB-5645: Independent reconstruction of Mm-cpn cryo-EM density map from half dataset in the closed state (Training map)  EMDB-5646: |
| Source |
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 Keywords |  CHAPERONE / modeling / independent reconstruction / cryo-EM model validation |
