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TitleIdentification of the outer-inner dynein linker as a hub controller for axonemal dynein activities.
Journal, issue, pagesCurr Biol, Vol. 23, Issue 8, Page 656-664, Year 2013
Publish dateApr 22, 2013
AuthorsToshiyuki Oda / Toshiki Yagi / Haruaki Yanagisawa / Masahide Kikkawa /
PubMed AbstractBACKGROUND: In flagella, the outer dynein arm (ODA) and inner dynein arm (IDA) play distinct roles in generating beating motion. However, functional communications between the two dyneins have not ...BACKGROUND: In flagella, the outer dynein arm (ODA) and inner dynein arm (IDA) play distinct roles in generating beating motion. However, functional communications between the two dyneins have not been investigated.
RESULTS: Here, we demonstrated by cryo-electron microscopy and chemical crosslinking that intermediate chain 2 (IC2) of ODAs interacts with the dynein regulatory complex in the axoneme and constitutes part of the outer-inner dynein (OID) linker. Furthermore, we identified IC2 as a functional hub between ODAs and IDAs based on the phenotypes of Chlamydomonas mutants expressing biotinylation-tagged IC2. The flagella of the IC2 mutant showed activated microtubule sliding and enhanced ATPase activities of ODAs, as well as an altered waveform, indicating attenuated IDA activity.
CONCLUSIONS: We concluded that the OID linker controls both ODAs and IDAs and regulates flagellar beating.
External linksCurr Biol / PubMed:23583547
MethodsEM (single particle)
Resolution34.0 Å
Structure data

EMDB-5618:
Outer dynein arm - microtubule complex
Method: EM (single particle) / Resolution: 34.0 Å

EMDB-5619:
Outer dynein arm - microtubule complex, labeled with biotin-ADP and streptavidin
Method: EM (single particle) / Resolution: 34.0 Å

EMDB-5620:
Outer dynein arm - microtubule complex, biotin-streptavidin tagged at the amino-terminus of the intermediate chain 2.
Method: EM (single particle) / Resolution: 34.0 Å

EMDB-5621:
Outer dynein arm - microtubule complex, biotin-streptavidin tagged at the carboxyl-terminus of the intermediate chain 2.
Method: EM (single particle) / Resolution: 34.0 Å

EMDB-5622:
Outer dynein arm - microtubule complex, biotin-streptavidin tagged at the amino-terminus of the intermediate chain 1.
Method: EM (single particle) / Resolution: 34.0 Å

Source
  • Chlamydomonas reinhardtii (plant)

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