+検索条件
-Structure paper
タイトル | A pseudoatomic model of the COPII cage obtained from cryo-electron microscopy and mass spectrometry. |
---|---|
ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 20, Issue 2, Page 167-173, Year 2013 |
掲載日 | 2012年12月23日 |
![]() | Alex J Noble / Qian Zhang / Jason O'Donnell / Hanaa Hariri / Nilakshee Bhattacharya / Alan G Marshall / Scott M Stagg / ![]() |
PubMed 要旨 | COPII vesicles transport proteins from the endoplasmic reticulum to the Golgi apparatus. Previous COPII-cage cryo-EM structures lacked the resolution necessary to determine the residues of Sec13 and ...COPII vesicles transport proteins from the endoplasmic reticulum to the Golgi apparatus. Previous COPII-cage cryo-EM structures lacked the resolution necessary to determine the residues of Sec13 and Sec31 that mediate assembly and flexibility of the COPII cage. Here we present a 12-Å structure of the human COPII cage, where the tertiary structure of Sec13 and Sec31 is clearly identifiable. We employ this structure and a homology model of the Sec13-Sec31 complex to create a reliable pseudoatomic model of the COPII cage. We combined this model with hydrogen/deuterium-exchange MS analysis to characterize four distinct contact regions at the vertices of the COPII cage. Furthermore, we found that the two-fold symmetry of the Sec31 dimeric region in Sec13-Sec31 is broken upon cage formation and that the resulting hinge is essential to form the proper edge geometry in COPII cages. |
![]() | ![]() ![]() ![]() |
手法 | EM (単粒子) |
解像度 | 12.0 Å |
構造データ | ![]() EMDB-5524: |
由来 |
|