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TitleCryo-EM structure of a 3D DNA-origami object.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 109, Issue 49, Page 20012-20017, Year 2012
Publish dateDec 4, 2012
AuthorsXiao-Chen Bai / Thomas G Martin / Sjors H W Scheres / Hendrik Dietz /
PubMed AbstractA key goal for nanotechnology is to design synthetic objects that may ultimately achieve functionalities known today only from natural macromolecular complexes. Molecular self-assembly with DNA has ...A key goal for nanotechnology is to design synthetic objects that may ultimately achieve functionalities known today only from natural macromolecular complexes. Molecular self-assembly with DNA has shown potential for creating user-defined 3D scaffolds, but the level of attainable positional accuracy has been unclear. Here we report the cryo-EM structure and a full pseudoatomic model of a discrete DNA object that is almost twice the size of a prokaryotic ribosome. The structure provides a variety of stable, previously undescribed DNA topologies for future use in nanotechnology and experimental evidence that discrete 3D DNA scaffolds allow the positioning of user-defined structural motifs with an accuracy that is similar to that observed in natural macromolecules. Thereby, our results indicate an attractive route to fabricate nanoscale devices that achieve complex functionalities by DNA-templated design steered by structural feedback.
External linksProc Natl Acad Sci U S A / PubMed:23169645 / PubMed Central
MethodsEM (single particle)
Resolution11.5 Å
Structure data

2210

4v5x

EMDB-2210, PDB-4v5x:
The cryo-EM structure of a 3D DNA-origami object
Method: EM (single particle) / Resolution: 11.5 Å

Source
  • enterobacteria phage m13 (virus)
  • synthetic construct (others)
KeywordsDNA / DNA-TEMPLATED DESIGN / SYNTHETIC BIOLOGY

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