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TitleA Bacterial Glycosidase Enables Mannose-6-Phosphate Modification and Improved Cellular Uptake of Yeast-Produced Recombinant Human Lysosomal Enzymes.
Journal, issue, pagesNat. Biotechnol., Vol. 30, Page 1225-, Year 2012
Publish dateSep 29, 2010 (structure data deposition date)
AuthorsTiels, P. / Baranova, E. / Piens, K. / De Visscher, C. / Pynaert, G. / Nerinckx, W. / Stout, J. / Fudalej, F. / Hulpiau, P. / Tannler, S. ...Tiels, P. / Baranova, E. / Piens, K. / De Visscher, C. / Pynaert, G. / Nerinckx, W. / Stout, J. / Fudalej, F. / Hulpiau, P. / Tannler, S. / Geysens, S. / Van Hecke, A. / Valevska, A. / Vervecken, W. / Remaut, H. / Callewaert, N.
External linksNat. Biotechnol. / PubMed:23159880
MethodsX-ray diffraction
Resolution2 - 2.09 Å
Structure data

PDB-2xsg:
Structure of the gh92 family glycosyl hydrolase ccman5
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4aq0:
Structure of the Gh92 Family Glycosyl Hydrolase Ccman5 in complex with deoxymannojirimycin
Method: X-RAY DIFFRACTION / Resolution: 2.09 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-B3P:
2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-DMJ:
1-DEOXYMANNOJIRIMYCIN

Source
  • cellulosimicrobium cellulans (bacteria)
KeywordsHYDROLASE / MANNOSIDASE

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