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TitleRational Design, Synthesis, Evaluation and Enzyme-Substrate Structures of Improved Fluorogenic Substrates for Family 6 Glycoside Hydrolases.
Journal, issue, pagesFEBS J., Vol. 280, Page 184-, Year 2013
Publish dateMay 11, 2012 (structure data deposition date)
AuthorsWu, M. / Nerinckx, W. / Piens, K. / Ishida, T. / Hansson, H. / Sandgren, M. / Stahlberg, J.
External linksFEBS J. / PubMed:23137336
MethodsX-ray diffraction
Resolution1.7 - 2.3 Å
Structure data

PDB-4au0:
Hypocrea jecorina Cel6A D221A mutant soaked with 6-chloro-4- methylumbelliferyl-beta-cellobioside
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-4ax6:
HYPOCREA JECORINA CEL6A D221A MUTANT SOAKED WITH 6-CHLORO-4- PHENYLUMBELLIFERYL-BETA-CELLOBIOSIDE
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-4ax7:
Hypocrea jecorina Cel6A D221A mutant soaked with 4-Methylumbelliferyl- beta-D-cellobioside
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-MAN:
alpha-D-mannopyranose

ChemComp-XZZ:
6-chloro-7-hydroxy-4-methyl-2H-chromen-2-one

ChemComp-BGC:
beta-D-glucopyranose

ChemComp-HOH:
WATER

ChemComp-UWU:
6-chloranyl-7-oxidanyl-4-phenyl-chromen-2-one

ChemComp-4MU:
7-hydroxy-4-methyl-2H-chromen-2-one

Source
  • hypocrea jecorina (fungus)
  • trichoderma reesei (fungus)
KeywordsHYDROLASE / HYDROLASE(O-GLYCOSYL) / GLYCOSIDASE / GLYCOSIDE HYDROLASE / GH6 / CLMUFG2 / CELLULASE / GLYCOPROTEIN / FLUOROGENIC SUBSTRATE / CLPHUFG2 / CELLOBIOHYDROLASE / MUFG2

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