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TitleStructural and mechanistic insights into guanylylation of RNA-splicing ligase RtcB joining RNA between 3'-terminal phosphate and 5'-OH.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 109, Page 15235-15240, Year 2012
Publish dateFeb 26, 2012 (structure data deposition date)
AuthorsEnglert, M. / Xia, S. / Okada, C. / Nakamura, A. / Tanavde, V. / Yao, M. / Eom, S.H. / Konigsberg, W.H. / Soll, D. / Wang, J.
External linksProc. Natl. Acad. Sci. USA / PubMed:22949672
MethodsX-ray diffraction
Resolution1.48 - 2.25 Å
Structure data

PDB-4dwq:
RNA ligase RtcB-GMP/Mn(2+) complex
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

PDB-4dwr:
RNA ligase RtcB/Mn2+ complex
Method: X-RAY DIFFRACTION / Resolution: 1.48 Å

Chemicals

ChemComp-5GP:
GUANOSINE-5'-MONOPHOSPHATE

ChemComp-MN:
Unknown entry

ChemComp-MLI:
MALONATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-PO4:
PHOSPHATE ION

ChemComp-POP:
PYROPHOSPHATE 2-

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

Source
  • pyrococcus horikoshii (archaea)
KeywordsLIGASE / RtcB / guanylylation / RNA ligase / RtcB-GMP / Mn2+

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