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TitleAcetylcholine-binding protein in the hemolymph of the planorbid snail Biomphalaria glabrata is a pentagonal dodecahedron (60 subunits).
Journal, issue, pagesPLoS One, Vol. 7, Issue 8, Page e43685, Year 2012
Publish dateAug 20, 2012
AuthorsMichael Saur / Vanessa Moeller / Katharina Kapetanopoulos / Sandra Braukmann / Wolfgang Gebauer / Stefan Tenzer / Jürgen Markl /
PubMed AbstractNicotinic acetylcholine receptors (nAChR) play important neurophysiological roles and are of considerable medical relevance. They have been studied extensively, greatly facilitated by the gastropod ...Nicotinic acetylcholine receptors (nAChR) play important neurophysiological roles and are of considerable medical relevance. They have been studied extensively, greatly facilitated by the gastropod acetylcholine-binding proteins (AChBP) which represent soluble structural and functional homologues of the ligand-binding domain of nAChR. All these proteins are ring-like pentamers. Here we report that AChBP exists in the hemolymph of the planorbid snail Biomphalaria glabrata (vector of the schistosomiasis parasite) as a regular pentagonal dodecahedron, 22 nm in diameter (12 pentamers, 60 active sites). We sequenced and recombinantly expressed two ∼25 kDa polypeptides (BgAChBP1 and BgAChBP2) with a specific active site, N-glycan site and disulfide bridge variation. We also provide the exon/intron structures. Recombinant BgAChBP1 formed pentamers and dodecahedra, recombinant BgAChBP2 formed pentamers and probably disulfide-bridged di-pentamers, but not dodecahedra. Three-dimensional electron cryo-microscopy (3D-EM) yielded a 3D reconstruction of the dodecahedron with a resolution of 6 Å. Homology models of the pentamers docked to the 6 Å structure revealed opportunities for chemical bonding at the inter-pentamer interfaces. Definition of the ligand-binding pocket and the gating C-loop in the 6 Å structure suggests that 3D-EM might lead to the identification of functional states in the BgAChBP dodecahedron.
External linksPLoS One / PubMed:22916297 / PubMed Central
MethodsEM (single particle)
Resolution6.0 Å
Structure data

2055

4aod

4aoe

EMDB-2055: Acetylcholine-binding protein in the hemolymph of the planorbid snail Biomphalaria glabrata is a pentagonal dodecahedron (60 subunits)
PDB-4aod: Biomphalaria glabrata Acetylcholine-binding protein type 1 (BgAChBP1)
PDB-4aoe: Biomphalaria glabrata Acetylcholine-binding protein type 2 (BgAChBP2)
Method: EM (single particle) / Resolution: 6.0 Å

Source
  • biomphalaria glabrata (bloodfluke planorb)
KeywordsACETYLCHOLINE-BINDING PROTEIN / LIGAND GATED ION CHANNEL / LGIC / CYS-LOOP RECEPTOR / ACHBP / ACETYLCHOLINE BINDING PROTEIN / ACETYLCHOLINE / ACHR / ACETYLCHOLINE RECEPTOR / MYASTHENIA GRAVIS / NICOTINIC / DODECAHEDRON / SCHISTOSOMA MANSONI / BILHARZIOSIS / SNAIL / PENTAGONAL DODECAHEDRON

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