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Structure paper

TitleStructure of the rigor actin-tropomyosin-myosin complex.
Journal, issue, pagesCell, Vol. 150, Issue 2, Page 327-338, Year 2012
Publish dateJul 20, 2012
AuthorsElmar Behrmann / Mirco Müller / Pawel A Penczek / Hans Georg Mannherz / Dietmar J Manstein / Stefan Raunser /
PubMed AbstractRegulation of myosin and filamentous actin interaction by tropomyosin is a central feature of contractile events in muscle and nonmuscle cells. However, little is known about molecular interactions ...Regulation of myosin and filamentous actin interaction by tropomyosin is a central feature of contractile events in muscle and nonmuscle cells. However, little is known about molecular interactions within the complex and the trajectory of tropomyosin movement between its "open" and "closed" positions on the actin filament. Here, we report the 8 Å resolution structure of the rigor (nucleotide-free) actin-tropomyosin-myosin complex determined by cryo-electron microscopy. The pseudoatomic model of the complex, obtained from fitting crystal structures into the map, defines the large interface involving two adjacent actin monomers and one tropomyosin pseudorepeat per myosin contact. Severe forms of hereditary myopathies are linked to mutations that critically perturb this interface. Myosin binding results in a 23 Å shift of tropomyosin along actin. Complex domain motions occur in myosin, but not in actin. Based on our results, we propose a structural model for the tropomyosin-dependent modulation of myosin binding to actin.
External linksCell / PubMed:22817895 / PubMed Central
MethodsEM (helical sym.) / EM (single particle)
Resolution7.7 - 8.9 Å
Structure data

1987

4a7f

4a7h

EMDB-1987, PDB-4a7f:
Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 3)
PDB-4a7h: Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 2)
Method: EM (helical sym.) / Resolution: 7.7 Å

1988

4a7h

EMDB-1988, PDB-4a7h:
Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 2)
Method: EM (helical sym.) / Resolution: 7.8 Å

1989

4a7l

EMDB-1989, PDB-4a7l:
Structure of the Actin-Tropomyosin-Myosin Complex (rigor ATM 1)
Method: EM (helical sym.) / Resolution: 8.1 Å

1990

4a7n

EMDB-1990, PDB-4a7n:
Structure of bare F-actin filaments obtained from the same sample as the Actin-Tropomyosin-Myosin Complex
Method: EM (helical sym.) / Resolution: 8.9 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-CA:
Unknown entry

Source
  • oryctolagus cuniculus (rabbit)
  • Gallus gallus (chicken)
  • dictyostelium discoideum (eukaryote)
KeywordsSTRUCTURAL PROTEIN/HYDROLASE / STRUCTURAL PROTEIN-HYDROLASE COMPLEX / STRUCTURAL PROTEIN / CYTOSKELETON / CONTRACTILE FILAMENT / MOTOR ACTIVITY / MYOSIN BINDING / ACTIN BINDING / ATP CATABOLIC PROCESS / RIGOR STATE

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