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Structure paper

Title5'-Cytosine-Phosphoguanine (Cpg) Methylation Impacts the Activity of Natural and Engineered Meganucleases.
Journal, issue, pagesJ. Biol. Chem., Vol. 287, Page 30139-, Year 2012
Publish dateApr 19, 2012 (structure data deposition date)
AuthorsValton, J. / Daboussi, F. / Leduc, S. / Molina, R. / Redondo, P. / Macmaster, R. / Montoya, G. / Duchateau, P.
External linksJ. Biol. Chem. / PubMed:22740697
MethodsX-ray diffraction
Resolution2.2 - 2.3 Å
Structure data

PDB-4aqu:
Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of calcium
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-4aqx:
Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of magnesium
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

Source
  • chlamydomonas reinhardtii (plant)
  • synthetic construct (others)
KeywordsHYDROLASE / GENE TARGETING / PROTEIN-DNA INTERACTION / HOMING ENDONUCLEASES / METHYLATION / GENETICS

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