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TitleStructural flexibility of RNA as molecular basis for Hfq chaperone function.
Journal, issue, pagesNucleic Acids Res, Vol. 40, Issue 16, Page 8072-8084, Year 2012
Publish dateJun 19, 2012
AuthorsEuripedes de Almeida Ribeiro / Mads Beich-Frandsen / Petr V Konarev / Weifeng Shang / Branislav Vecerek / Georg Kontaxis / Hermann Hämmerle / Herwig Peterlik / Dmitri I Svergun / Udo Bläsi / Kristina Djinović-Carugo /
PubMed AbstractIn enteric bacteria, many small regulatory RNAs (sRNAs) associate with the RNA chaperone host factor Q (Hfq) and often require the protein for regulation of target mRNAs. Previous studies suggested ...In enteric bacteria, many small regulatory RNAs (sRNAs) associate with the RNA chaperone host factor Q (Hfq) and often require the protein for regulation of target mRNAs. Previous studies suggested that the hexameric Escherichia coli Hfq (Hfq(Ec)) binds sRNAs on the proximal site, whereas the distal site has been implicated in Hfq-mRNA interactions. Employing a combination of small angle X-ray scattering, nuclear magnetic resonance and biochemical approaches, we report the structural analysis of a 1:1 complex of Hfq(Ec) with a 34-nt-long subsequence of a natural substrate sRNA, DsrA (DsrA(34)). This sRNA is involved in post-transcriptional regulation of the E. coli rpoS mRNA encoding the stationary phase sigma factor RpoS. The molecular envelopes of Hfq(Ec) in complex with DsrA(34) revealed an overall asymmetric shape of the complex in solution with the protein maintaining its doughnut-like structure, whereas the extended DsrA(34) is flexible and displays an ensemble of different spatial arrangements. These results are discussed in terms of a model, wherein the structural flexibility of RNA ligands bound to Hfq stochastically facilitates base pairing and provides the foundation for the RNA chaperone function inherent to Hfq.
External linksNucleic Acids Res / PubMed:22718981 / PubMed Central
MethodsSAS (X-ray synchrotron)
Structure data

SASDAH5:
Complex of Hfq with DsrA (RNA chaperone Hfq, Hfq + RNA DsrA, Hfq_DsrA)
Method: SAXS/SANS

Source
  • Escherichia coli (E. coli)

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