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Structure paper

TitleCsy4 Relies on an Unusual Catalytic Dyad to Position and Cleave Crispr RNA.
Journal, issue, pagesEmbo J., Vol. 31, Page 2824-, Year 2012
Publish dateMar 2, 2012 (structure data deposition date)
AuthorsHaurwitz, R.E. / Sternberg, S.H. / Doudna, J.A.
External linksEmbo J. / PubMed:22522703
MethodsX-ray diffraction
Resolution2 - 2.63 Å
Structure data

PDB-4al5:
Crystal structure of the Csy4-crRNA product complex
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4al6:
Crystal structure of the S148ACsy4-crRNA complex
Method: X-RAY DIFFRACTION / Resolution: 2.63 Å

PDB-4al7:
Crystal structure of the Csy4-minimal crRNA complex
Method: X-RAY DIFFRACTION / Resolution: 2.32 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • pseudomonas aeruginosa (bacteria)
KeywordsHYDROLASE/RNA / HYDROLASE-RNA COMPLEX / CRISPR

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