EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	ATP-triggered conformational changes delineate substrate-binding and -folding mechanics of the GroEL chaperonin.
ジャーナル・号・ページ	Cell, Vol. 149, Issue 1, Page 113-123, Year 2012
掲載日	2012年3月30日
著者	Daniel K Clare / Daven Vasishtan / Scott Stagg / Joel Quispe / George W Farr / Maya Topf / Arthur L Horwich / Helen R Saibil /
PubMed 要旨	The chaperonin GroEL assists the folding of nascent or stress-denatured polypeptides by actions of binding and encapsulation. ATP binding initiates a series of conformational changes triggering the ...The chaperonin GroEL assists the folding of nascent or stress-denatured polypeptides by actions of binding and encapsulation. ATP binding initiates a series of conformational changes triggering the association of the cochaperonin GroES, followed by further large movements that eject the substrate polypeptide from hydrophobic binding sites into a GroES-capped, hydrophilic folding chamber. We used cryo-electron microscopy, statistical analysis, and flexible fitting to resolve a set of distinct GroEL-ATP conformations that can be ordered into a trajectory of domain rotation and elevation. The initial conformations are likely to be the ones that capture polypeptide substrate. Then the binding domains extend radially to separate from each other but maintain their binding surfaces facing the cavity, potentially exerting mechanical force upon kinetically trapped, misfolded substrates. The extended conformation also provides a potential docking site for GroES, to trigger the final, 100° domain rotation constituting the "power stroke" that ejects substrate into the folding chamber.
リンク	Cell / PubMed:22445172 / PubMed Central
手法	EM (単粒子)
解像度	7.0 - 8.5 Å
構造データ	EMDB-1997: ATP-triggered molecular mechanics of the chaperonin GroEL 手法: EM (単粒子) / 解像度: 7.0 Å 1998 4aaq EMDB-1998, PDB-4aaq: ATP-triggered molecular mechanics of the chaperonin GroEL 手法: EM (単粒子) / 解像度: 8.0 Å 1999 4aar EMDB-1999, PDB-4aar: ATP-triggered molecular mechanics of the chaperonin GroEL 手法: EM (単粒子) / 解像度: 8.0 Å 2000 4aas EMDB-2000, PDB-4aas: ATP-triggered molecular mechanics of the chaperonin GroEL 手法: EM (単粒子) / 解像度: 8.5 Å 2001 4aau EMDB-2001, PDB-4aau: ATP-triggered molecular mechanics of the chaperonin GroEL 手法: EM (単粒子) / 解像度: 8.5 Å 2002 4ab2 EMDB-2002, PDB-4ab2: ATP-triggered molecular mechanics of the chaperonin GroEL 手法: EM (単粒子) / 解像度: 8.5 Å 2003 4ab3 EMDB-2003, PDB-4ab3: ATP-triggered molecular mechanics of the chaperonin GroEL 手法: EM (単粒子) / 解像度: 8.5 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-PO4: PHOSPHATE ION / ホスファ-ト ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	escherichia coli (大腸菌) synthetic construct (人工物)
キーワード	CHAPERONE