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Structure paper

TitleThe use of trimethylamine N-oxide as a primary precipitating agent and related methylamine osmolytes as cryoprotective agents for macromolecular crystallography.
Journal, issue, pagesActa Crystallogr. ,Sect. D, Vol. 68, Page 69-81, Year 2012
Publish dateJul 22, 2011 (structure data deposition date)
AuthorsMarshall, H. / Venkat, M. / Hti Lar Seng, N.S. / Cahn, J. / Juers, D.H.
External linksActa Crystallogr. ,Sect. D / PubMed:22194335
MethodsX-ray diffraction
Resolution1.7 - 2.8 Å
Structure data

PDB-3t25:
TMAO-grown orthorhombic trypsin (bovine)
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-3t26:
Orthorhombic trypsin (bovine) in the presence of sarcosine
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-3t27:
Orthorhombic trypsin (bovine) in the presence of betaine
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-3t28:
TMAO-grown trypsin (bovine)-previously unreported tetragonal crystal form
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-3t29:
TMAO-grown trigonal trypsin (bovine)
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-3t2a:
TMAO-grown cubic insulin (porcine)
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-3t2h:
Tetragonal thermolysin in the presence of TMAO
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-3t2i:
Tetragonal thermolysin in the presence of sarcosine
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-3t2j:
Tetragonal thermolysin in the presence of betaine
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-SO4:
SULFATE ION

ChemComp-BEN:
BENZAMIDINE

ChemComp-TMO:
trimethylamine oxide

ChemComp-HOH:
WATER

ChemComp-SAR:
SARCOSINE

ChemComp-BET:
TRIMETHYL GLYCINE

ChemComp-ZN:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-DMS:
DIMETHYL SULFOXIDE / DMSO, precipitant*YM

Source
  • bos taurus (domestic cattle)
  • sus scrofa (pig)
  • bacillus thermoproteolyticus (bacteria)
KeywordsHYDROLASE / Protease / HORMONE / alpha/beta / Zinc Protease

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