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Structure paper

TitleStructural basis of tRNA agmatinylation essential for AUA codon decoding
Journal, issue, pagesNat. Struct. Mol. Biol., Vol. 18, Page 1275-1280, Year 2011
Publish dateAug 23, 2010 (structure data deposition date)
AuthorsOsawa, T. / Kimura, S. / Terasaka, N. / Inanaga, H. / Suzuki, T. / Numata, T.
External linksNat. Struct. Mol. Biol. / PubMed:22002223
MethodsX-ray diffraction
Resolution2.6 - 3.1 Å
Structure data

PDB-3amt:
Crystal structure of the TiaS-tRNA(Ile2)-ATP complex
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-3amu:
Crystal structure of the TiaS-tRNA(Ile2)-AMPCPP-agmatine complex
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

PDB-3au7:
Crystal structure of the ZRD-deleted mutant of TiaS in complex with agmatine
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-APC:
DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER / AMP-CPP, energy-carrying molecule analogue*YM

ChemComp-AG2:
AGMATINE

ChemComp-HOH:
WATER

Source
  • archaeoglobus fulgidus (archaea)
KeywordsRNA BINDING PROTEIN/RNA / TiaS / tRNA(Ile2) / modification / RNA BINDING PROTEIN-RNA complex / RNA BINDING PROTEIN / ATP hydrolysis

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