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TitleLow-Temperature Chromophore Isomerization Reveals the Photoswitching Mechanism of the Fluorescent Protein Padron.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 133, Page 16362-, Year 2011
Publish dateJul 18, 2011 (structure data deposition date)
AuthorsRegis Faro, A. / Carpentier, P. / Jonasson, G. / Pompidor, G. / Arcizet, D. / Demachy, I. / Bourgeois, D.
External linksJ. Am. Chem. Soc. / PubMed:21923132
MethodsX-ray diffraction
Resolution2.2 - 2.345 Å
Structure data

PDB-3zuf:
Padron off (non-fluorescent) Btrans
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-3zuj:
Padron on (fluorescent) ABcis
Method: X-RAY DIFFRACTION / Resolution: 2.345 Å

PDB-3zul:
Padron on (fluorescent) Icis intermediate state
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • echinophyllia sp. sc22 (invertebrata)
KeywordsFLUORESCENT PROTEIN / GFP VARIANTS / POSITIVE PHOTOSWITCHING / FPS / RSFPS / CRYO-PROBE / INTERMEDIATE STATE / ISOMERIZATION / POSITIVE / PHOTOSWITCHING

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