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TitleStructural analysis of the Ras-like G protein MglA and its cognate GAP MglB and implications for bacterial polarity.
Journal, issue, pagesEmbo J., Vol. 30, Page 4185-4197, Year 2011
Publish dateJul 21, 2011 (structure data deposition date)
AuthorsMiertzschke, M. / Koerner, C. / Vetter, I.R. / Keilberg, D. / Hot, E. / Leonardy, S. / Sogaard-Andersen, L. / Wittinghofer, A.
External linksEmbo J. / PubMed:21847100
MethodsX-ray diffraction
Resolution1.67 - 2.91 Å
Structure data

PDB-3t12:
MglA in complex with MglB in transition state
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-3t1o:
MglA bound to GDP
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-3t1q:
MglA bound to GppNHp in complex with MglB
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-3t1r:
MglB with tetrameric arrangement
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-3t1s:
MglB Homodimer
Method: X-RAY DIFFRACTION / Resolution: 1.67 Å

PDB-3t1t:
MglA bound to GDP in P1 tetrameric arrangement
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-3t1v:
MglA bound to GDP in P2(1) tetrameric arrangement
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-3t1x:
MglB R124A E127A Monomer
Method: X-RAY DIFFRACTION / Resolution: 2.91 Å

Chemicals

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-ALF:
TETRAFLUOROALUMINATE ION

ChemComp-HOH:
WATER

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-GNP:
PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / GppNHp, GMPPNP, energy-carrying molecule analogue*YM

Source
  • thermus thermophilus (bacteria)
KeywordsHYDROLASE/SIGNALING PROTEIN / G-domain containing protein / Bacterial Polarity / Motility / Homodimeric GAP protein / Pole localisation / alpha/beta proteins / GTPase / HYDROLASE-SIGNALING PROTEIN complex / HYDROLASE / G domain containing protein / bacterial GTPase / alpha/beta protein / GTPase activating protein / homodimer / SIGNALING PROTEIN / Bacterial Polarity and Motility / signalling protein / catalytic GAP domain / G domain containg protein

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