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TitleRibosome assembly factors prevent premature translation initiation by 40S assembly intermediates.
Journal, issue, pagesScience, Vol. 333, Issue 6048, Page 1449-1453, Year 2011
Publish dateSep 9, 2011
AuthorsBethany S Strunk / Cherisse R Loucks / Min Su / Harish Vashisth / Shanshan Cheng / Justin Schilling / Charles L Brooks / Katrin Karbstein / Georgios Skiniotis /
PubMed AbstractRibosome assembly in eukaryotes requires approximately 200 essential assembly factors (AFs) and occurs through ordered events that initiate in the nucleolus and culminate in the cytoplasm. Here, we ...Ribosome assembly in eukaryotes requires approximately 200 essential assembly factors (AFs) and occurs through ordered events that initiate in the nucleolus and culminate in the cytoplasm. Here, we present the electron cryo-microscopy (cryo-EM) structure of a late cytoplasmic 40S ribosome assembly intermediate from Saccharomyces cerevisiae at 18 angstrom resolution. We obtained cryo-EM reconstructions of preribosomal complexes lacking individual components to define the positions of all seven AFs bound to this intermediate. These late-binding AFs are positioned to prevent each step in the translation initiation pathway. Together, they obstruct the binding sites for initiation factors, prevent the opening of the messenger RNA channel, block 60S subunit joining, and disrupt the decoding site. These redundant mechanisms probably ensure that pre-40S particles do not enter the translation pathway, which would result in their rapid degradation.
External linksScience / PubMed:21835981 / PubMed Central
MethodsEM (single particle)
Resolution18.0 - 30.0 Å
Structure data

EMDB-1922:
Ribosome Assembly Factors Prevent Premature Translation Initiation by 40S Assembly Intermediates
Method: EM (single particle) / Resolution: 30.0 Å

EMDB-1923:
Ribosome Assembly Factors Prevent Premature Translation Initiation by 40S Assembly Intermediates
Method: EM (single particle) / Resolution: 20.0 Å

EMDB-1924:
Ribosome Assembly Factors Prevent Premature Translation Initiation by 40S Assembly Intermediates
Method: EM (single particle) / Resolution: 20.0 Å

EMDB-1925:
Ribosome Assembly Factors Prevent Premature Translation Initiation by 40S Assembly Intermediates
Method: EM (single particle) / Resolution: 22.0 Å

EMDB-1926:
Ribosome Assembly Factors Prevent Premature Translation Initiation by 40S Assembly Intermediates
Method: EM (single particle) / Resolution: 26.0 Å

EMDB-1927:
Ribosome Assembly Factors Prevent Premature Translation Initiation by 40S Assembly Intermediates
Method: EM (single particle) / Resolution: 18.0 Å

Source
  • Saccharomyces cerevisiae (brewer's yeast)

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