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Structure paper

TitleSnapshots of Enzymatic Baeyer-Villiger Catalysis: Oxygen Activation and Intermediate Stabilization.
Journal, issue, pagesJ. Biol. Chem., Vol. 286, Page 29284-, Year 2011
Publish dateJun 6, 2011 (structure data deposition date)
AuthorsOrru, R. / Dudek, H.M. / Martinoli, C. / Torres Pazmino, D.E. / Royant, A. / Weik, M. / Fraaije, M.W. / Mattevi, A.
External linksJ. Biol. Chem. / PubMed:21697090
MethodsX-ray diffraction
Resolution2 - 2.9 Å
Structure data

PDB-2ylr:
SNAPSHOTS OF ENZYMATIC BAEYER-VILLIGER CATALYSIS: OXYGEN ACTIVATION AND INTERMEDIATE STABILIZATION: COMPLEX WITH NADP
Method: X-RAY DIFFRACTION / Resolution: 2.26 Å

PDB-2yls:
SNAPSHOTS OF ENZYMATIC BAEYER-VILLIGER CATALYSIS: OXYGEN ACTIVATION AND INTERMEDIATE STABILIZATION: REDUCED ENZYME BOUND TO NADP
Method: X-RAY DIFFRACTION / Resolution: 2.26 Å

PDB-2ylt:
SNAPSHOTS OF ENZYMATIC BAEYER-VILLIGER CATALYSIS: OXYGEN ACTIVATION AND INTERMEDIATE STABILIZATION: COMPLEX WITH NADP and MES
Method: X-RAY DIFFRACTION / Resolution: 2.65 Å

PDB-2ylw:
SNAPSHOTS OF ENZYMATIC BAEYER-VILLIGER CATALYSIS: OXYGEN ACTIVATION AND INTERMEDIATE STABILIZATION: Arg337Lys MUTANT IN COMPLEX WITH MES
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-2ylx:
SNAPSHOTS OF ENZYMATIC BAEYER-VILLIGER CATALYSIS: OXYGEN ACTIVATION AND INTERMEDIATE STABILIZATION: Asp66Ala MUTANT IN COMPLEX WITH NADP AND MES
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-2ylz:
SNAPSHOTS OF ENZYMATIC BAEYER-VILLIGER CATALYSIS: OXYGEN ACTIVATION AND INTERMEDIATE STABILIZATION: Met446Gly MUTANT
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-2ym1:
SNAPSHOTS OF ENZYMATIC BAEYER-VILLIGER CATALYSIS: OXYGEN ACTIVATION AND INTERMEDIATE STABILIZATION: Arg337Lys MUTANT IN COMPLEX WITH NADP
Method: X-RAY DIFFRACTION / Resolution: 2.28 Å

PDB-2ym2:
SNAPSHOTS OF ENZYMATIC BAEYER-VILLIGER CATALYSIS: OXYGEN ACTIVATION AND INTERMEDIATE STABILIZATION: Arg337Lys MUTANT REDUCED STATE WITH NADP
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-HOH:
WATER

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-SO4:
SULFATE ION

ChemComp-OXY:
OXYGEN MOLECULE

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • thermobifida fusca (bacteria)
KeywordsOXIDOREDUCTASE / BAEYER-VILLIGER REACTION / FAD / OXYGENASE / BAEYER-VILLIGER

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