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TitleStructural and Biochemical Analyses of Regio- and Stereospecificities Observed in a Type II Polyketide Ketoreductase.
Journal, issue, pagesBiochemistry, Vol. 50, Page 4638-4649, Year 2011
Publish dateFeb 18, 2011 (structure data deposition date)
AuthorsJavidpour, P. / Korman, T.P. / Shakya, G. / Tsai, S.C.
External linksBiochemistry / PubMed:21506596
MethodsX-ray diffraction
Resolution2.292 - 2.792 Å
Structure data

PDB-3qrw:
Actinorhodin Polyketide Ketoreductase Mutant P94L bound to NADPH
Method: X-RAY DIFFRACTION / Resolution: 2.792 Å

PDB-3ri3:
Actinorhodin Polyketide Ketoreductase Mutant P94L bound to NADPH and the Inhibitor Emodin
Method: X-RAY DIFFRACTION / Resolution: 2.292 Å

Chemicals

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-FMT:
FORMIC ACID

ChemComp-HOH:
WATER

ChemComp-EMO:
3-METHYL-1,6,8-TRIHYDROXYANTHRAQUINONE

Source
  • streptomyces coelicolor (bacteria)
KeywordsOXIDOREDUCTASE / actinorhodin / polyketide / ketoreductase / short-chain dehydrogenase/reductase / Type II polyketide ketoreductase / Rossmann fold / OXIDOREDUCTASE/OXIDOREDUCTASE INHIBITOR / OXIDOREDUCTASE-OXIDOREDUCTASE INHIBITOR complex

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