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Structure paper

TitleResurrection of a functional phosphatidylinositol transfer protein from a pseudo-Sec14 scaffold by directed evolution.
Journal, issue, pagesMol. Biol. Cell, Vol. 22, Page 892-905, Year 2011
Publish dateJan 6, 2011 (structure data deposition date)
AuthorsSchaaf, G. / Dynowski, M. / Mousley, C.J. / Shah, S.D. / Yuan, P. / Winklbauer, E.M. / de Campos, M.K. / Trettin, K. / Quinones, M.C. / Smirnova, T.I. ...Schaaf, G. / Dynowski, M. / Mousley, C.J. / Shah, S.D. / Yuan, P. / Winklbauer, E.M. / de Campos, M.K. / Trettin, K. / Quinones, M.C. / Smirnova, T.I. / Yanagisawa, L.L. / Ortlund, E.A. / Bankaitis, V.A.
External linksMol. Biol. Cell / PubMed:21248202
MethodsX-ray diffraction
Resolution1.8 Å
Structure data

PDB-3q8g:
Resurrection of a functional phosphatidylinositol transfer protein from a pseudo-Sec14 scaffold by directed evolution
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

Chemicals

ChemComp-PEE:
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, phospholipid*YM

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsSIGNALING PROTEIN / CRAL-TRIO / string motif / directed evolution / PIPT / SEC14 / phospholipid transporter / lipid / phosphatidylinositol / phosphatidylcholine

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